MAN, MEALS and MYCOTOXINS

As humanity grows on daily basis, there is another group of small creatures called fungi, which are ubiquitous  and most of which cannot be seen with unaided eyes. The Nigerian climate support the growth of this group and the subsequent production of chemicals on food items in farm, transit and store. These chemicals are toxic and are referred to as fungal toxins or mycotoxins. These toxic chemical s are found as contaminants of many items including maize, wheat, beans, groundnut, milk and milk products, yam, cassava. It is instructive that toxin production is predicated on mould growth. Nearly a quarter of the global crops are affected by them and their consumption as dietary chemicals by man and livestock incites a range of morbidities and on few occasions, mortality. Four divisions are very important because of their potency  and  increased research attention and, therefore health and  international trade impacts. These are aflatoxins, ochratoxins, patulin and trichothecenes .  Let’s start with the first one aflatoxins. This is a group that harbours the only known toxin of biological origin that has been so recognized by the International Agency for Research on Cancer, IARC. In the late 80s the Agency that serves as a technical arm of the WHO, officially placed aflatoxin B1 on the list of Type A carcinogen. The interpretation of this classification is that aflatoxin can incite cancer in human. Indeed cases of aflatoxin-induced liver cancer and immune suppression have been reported in livestock and man. High temperature, humidity and economic challenge all combine to present a formidable predisposing package. The producing fungus, Aspergillus flavus , is very common in Nigerian soil. On stale food items,  it occurs as green coating , with  the toxin  representing  a product of its secondary metabolism. This metabolite, which  is colourless , odourless and tasteless,  comes as B and G both representing the dietary forms and M  being the one found in milk. Washing of the mouldy crop  food  will not remove the already secreted contaminating poison. It is heat stable so all forms of boiling may not provide  any intervention. The temperature adequate to destroy it is equally sufficient the food item, so there will not be any net gain  using heat to treat aflatoxin-contaminated food item. National , Continental and global bodies have been formed to address arising alimentary and export concerns.

The Mycotoxicology Society of Nigeria , Partnership for Aflatoxin   Control in Africa , PACA and the International Society for Mycotoxicology are various initiatives put in place to recommend and possibly implement interventions. At present, The MSN has its secretariat at NAFDAC, Lagos where it  attends to public enquiries from the food and livestock industries.

As you read this know that you have a role to play in achieving health through wholesome  ‘mealing’  Please tell your neighbour……. and even your enemy  !!!

Let s assist the UN in realizing the goals on Food safety as captured in the SDGs

 

 

DF

Chromogenic Detection –Food Microbiology in Colour

In testing food samples for microbial contamination, mandatory subcultures and confirmatory biochemical tests make the procedure sometimes cumbersome, taking a longer time. However, a RAPID  and ACCURATE means of detection and enumerating microorganisms presents a faster and more reliable method. It uses colour production by the desired isolate, based on the specific enzyme activities

Lab M is a notable manufacturer of these media. Among the tested media are Listeria monocytogenes, coliforms and E coli  0157:H7 and Salmonella species. As a matter of fact, Lab M’ Harlequin Listeria Chromogenic Agar was the first chromogenic dehydrated Listeria medium as specified by ISO 11290. Its reliable in food safety testing. There s also the one for Salmonella that equally proves  effective.  Harlequin Tryptone Bile Glucuronide Agar, TBGA,  was developed specifically  for  simple enumeration of E. coli without the need for membrane or pre-incubation

 

For more information, please contact LabM website

 

 

Manufacturers are encouraged  to send us updated information on their products. Just make sure it has relevance to FOOD SAFETY

 

 

Dele Fapohunda PhD

10 January 2023

FOOD SAFETY and MICROBIOLOGICAL QUALITY

FOOD SAFETY AND MICROBIOLOGICAL QUALITY

In the manufacture of food, cosmetics and pharmaceuticals,  it is necessary to ensure strict microbiological quality for it to be generally safe and acceptable. The procedure is important to be sure

of

 absence of pathogenic microbes

sterility, where desired

This is complemented by the monitoring of air and water quality using standard diagnostic measures. As a Public  Analyst, the production environment is very critical to the determination of product hygiene and quality. Therefore   quality attention ,  time and resources should be deployed in this direction

Microbiological Tests of interest include

1 Limit test=this is designed to determine the total aerobic , yeast and microbial counts. The test should reveal if the product is free from  Staphylococcus aureus,  Escherichia coli,  Pseudomonas aeruginosa, Salmonella, , Aspergillus niger and Candida albicans

  1. Sterility Testing=This is carried out under aseptic conditions. The Membrane Filtration method is acceptable in determining this. It ensures sterility where desired

3.Stability Testing=Generally used in assess the adequacy of the storage conditions. The Good Manufacturing Practice is handy in this respect

4.Toxicity Testing=  Can be done on experimental animals. Its only slightly reliable because it does not take into account the issue of  genetic variation

  1. Endotoxin Test=A very critical quality control test particularly at the final stages of drug formulation. Also useful in food safety testing

6Preservative Efficacy Testing=It is also called Antibiotics Potency Test. Antibiotics are added to the product(food , drug or cosmetics) to protect it  from contamination. Their survival can be monitored within 28 days. Five microbes are candidates for this test—E.coli, Staphylococcus aureus, Pseudomonas aeruginosa  Aspergillus niger and Candida albicans

 

 

Dele Fapohunda

10 Dec 2022

 

 

SAMPLING AND FOOD SAFETY

In carrying out food testing to arrive at ‘safe’ or ‘unsafe’, sampling must be done. It is the amount of effort deployed in sampling and sample preparation that determines the reliability of test result. False positive or false negative  may be arrived at if sampling is not properly done. This may lead to loss of money invested in the production and integrity/good will. The latter may not easily be recovered over time

Samples must be Representative I e  Ensuring homogeneity

Ensuring relative size of sample to be taken

Reliability and intended use of expected result

The data got may be applied in the following

A labelling requirement

B assurance of compliance with stated standards

C monitoring production as part of HACCP

D routine quality control

 

 

 

Dele Fapohunda

10 December, 2022

ANALYSIS AND FOOD SAFETY————-CHROMATOGRAPHY

This is a separation technique which is carried out according to size, solubility, boiling point and volatility.

Basic Terms

Analyte=substance/compound to be separated

Chromatogram=print out of the  showing different separated analytes

Chromatograph=the equipment that does the separation

Eluate=solution of solvent and dissolved matter collected from elution

Eluent=solvent that carries analytes

Stationary phase=a selected compound at a fixed place fro the procedure e g silica gel

The matrix (support) can be polar or non polar. It can preparative(to purify for further analysis) or analytical(to measure relative proportion of analytes in a mixture)

Types

1.Thin Layer Chromatography TLC

Applicable for the analysis of pesticides, food, fatty acids, food , phytochemicals and water. Adsorbent like silica gel can be spread as a thick slurry on the commercially available plates to a thickness of 0.1 -0.5mm(for analysis) and 0.5-2.0mm(for purity). Components of the sample move at different rates with respect to how they can bind onto the stationary phase. The movement is measured as Rf value , a figure that is specific to each analyte. Filter paper(cellulose) and silica gel are examples of stationary phase

A modification include HPTLC,  High Performance Thin Layer Chromatography

2.High Performance Liquid Chromatography HPLC

This is a separation based on the analytes relative solubility between 2 phases. Sample ca  eb loaded in 5-20 microliter volume. The components of the chromatogram are pump, injector, column, detector and computer. Its effective in separating pharmaceuticals, mycotoxins, salts, proteins, polystyrenes, heavy hydrocarbons, phytochemicals, and enzymes

3.Gas  Chromatography

Sometimes called Gas Liquid Chromatography

It is a requirement that samples must be vaporised without decay or decomposition. Mobile phase or carrier gas is Helium or Nitrogen. The stationary phase called a column, a metal tubing. The vaporised compound will elute at different times called RETENTION TIME, RT which is specific for each analyte and so used to identify them. As the chemicals go out of the column, they are identified electronically

Detectors are used in further elucidation of the analytes. These include UV, Fluorescence FID etc. Columns are also selected based on effectiveness. However, C-18 column is regarded as universal , capable of compatibility with a wide range of compounds.

 

 

 

dele Fapohunda

10 December, 2022

FOOD SAFETY and HAZARD ANALYSIS CRITICAL CONTROL POINTS, HACCP

A systematic preventive scheme to food safety. It also applies to pharmaceuticals,  cosmetics and related products. It is intended to address the physical, chemical and biological hazards. Its goal is to arrive at the best product desired. It is better than inspecting product after operations

It rests on 7 principles

1 Conduct a hazard analysis—identification of possible safety threats

2 Identify Critical Control Points, CCP

3 Establish critical limits for each CCP

4 Establish CCP monitoring requirements, to ensure the process is under control

5 Establish corrective action, to quickly address deviations

6.Establish record keeping procedure

7 Establish procedure for ensuring  the HACCP system is working—-through Validation

 

The overall implementation involves Monitoring, Verifying and Validation. The steps are contained in ISO 22000

 

 

Dele Fapohunda

10 December, 2022

 

FOOD SAFETY and HAZARD ANALYSIS CRITICAL CONTROL POINTS, HACCP

A systematic preventive scheme to food safety. It also applies to pharmaceuticals,  cosmetics and related products. It is intended to address the physical, chemical and biological hazards. Its goal is to arrive at the best product desired. It is better than inspecting product after operations

It rests on 7 principles

1 Conduct a hazard analysis—identification of possible safety threats

2 Identify Critical Control Points, CCP

3 Establish critical limits for each CCP

4 Establish CCP monitoring requirements, to ensure the process is under control

5 Establish corrective action, to quickly address deviations

6.Establish record keeping procedure

7 Establish procedure for ensuring  the HACCP system is working—-through Validation

 

The overall implementation involves Monitoring, Verifying and Validation. The steps are contained in ISO 22000

Dele Fapohunda

10 Dec 2022

AFLATOXIN and PESTICIDE ANALYSES NOW HERE

A validated laboratory that analyses dietary aflatoxins and a many pesticides is right here at your service. HPLC and GC are the equipment available for reliable detection and quantitation. The lab is under the leadership of an experienced Public Analyst

For further details, Please contact
sfoodfeedf@gmail.com
or 234 8033709492

FOOD SAFETY, AN EMERGING CULTURE IN AFRICA?

There is hardly a healthy report from the European Union concerning the status of contaminants in agric products exported from African countries . Lack of awareness and economic challenge are some of the risk factors that have opened the window for consumption of unwholesome food and feed . Credit mu8st be given to continental interventions like CAADP, FARA, ASM who have displayed credible passion on the crusade against unsafe food. From Senegal, through Tripoli and Nairobi, to Angola and South Africa, the story of near neglect is similar. Just last year 2020, the EU had to further extend the ban placed on Nigeria concerning her dried beans, this time to 2022. Although workshops and conferences are being held, it is clear that critical gaps and scary holes are still there to be filled.

Safe Food and Feed Foundation appreciates the effort of the organizers of the forthcoming Food safety  Summit  taking place in South Africa from June 8-9,2021 . Being a the second outing by the organizers, the ambition and passion are clear, but expected  beneficial result is the goal, where more and more Africans will now access safe food that will guarantee health and enhance export value.

After the Summit, there must be measured and time bound feed back and follow up. It is only after this is done that it can be separated from other hollow talk-shops !!!!

We call on other African countries to emulate this and make the development a more aggressive continental initiative that must attract the needed  urgency and importance from governments and policy makers

 

 

 

Dele Fapohunda

20 April, 2021

Mesothelioma cancer, the asbestos-driven silent killer

Mesothelioma Cancer – The Mesothelioma Center

Mesothelioma is a malignant tumor caused by inhaled asbestos fibers. It usually affects the lining of the lungs, however mesothelioma tumors can also form on the linings of the abdomen, heart, and testes.

Pleural mesothelioma is the most common form, accounting for 75% of all cases. It affects the lining of the lungs, and symptoms can include shortness of breath, chest pain, dry coughing and more. The second more common type is peritoneal mesothelioma, which forms in the lining of the abdomen. Although less common, other forms of mesothelioma include pericardial (heart), and testicular mesothelioma.

Asbestos exposure is the leading cause of mesothelioma. Asbestos is a group of six naturally occurring minerals composed of soft, flexible fibers. It was previously used in many consumer products due to its heat resistance.

Those at high risk of developing mesothelioma are blue-collar workers, veterans, and family members of those regularly exposed to asbestos fibers. Firefighters, construction workers, power plant workers, shipyard workers, and others are at high risk of occupational asbestos exposure. U.S. Navy veterans also face health risks due to the abundance of products that were used in many of the military products before the 1980’s.

Due to its long latency period, about 20-50 years, most patients are diagnosed in their late 60’s. Men also account for a majority of mesothelioma diagnoses.

Interventions for Mesothelioma can involve   clinical trials,   conventional cancer treatments such as chemotherapy, radiation, surgery as well as alternative therapies.

It is a silent killer with most cases generally ignorant. Are you exposed to asbestos regularly ? Then this may interest you

 

To find more information on mesothelioma, and it’s more common forms please visit the links below:

 

https://www.asbestos.com/mesothelioma/

https://www.asbestos.com/mesothelioma/pleural/

https://www.asbestos.com/mesothelioma/peritoneal/

 

Source: Mesothelioma Cancer Center, USA

 

The Safe Food and Feed Foundation fully aligns with the objectives of Mesothelioma Cancer Center, USA. For more info , please contact sfoodfeedf@gmail.com

@sfoodfeedf

 

Dele

28 Oct 2020